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plasmid more than recommended for transfection


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#1 Curtis

Curtis

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Excellent

Posted 28 August 2010 - 03:14 AM

Is higher amount of plasmid toxic to the cells in transfection? Why is there a limit for plasmid concentration in transfection?

Because I'm getting pretty better results when using more plasmid than recommended. I get >80% efficiency in 10cm well of HeLa cells when I use 10ug of pEGFP.

#2 Curtis

Curtis

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Excellent

Posted 05 September 2010 - 03:02 AM

anyone?




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