E. coli not growing in liquid cultures :(
Posted 27 August 2010 - 09:27 AM
I have a ligated mix from restriction digests which I transform into XL2 Blue cells. I select colonies by blue-white color screening on LB-Amp plates containing X-Gal and IPTG. At this point I assume that my ligation product would be found in the white colonies so I pick a few and I inoculate 5mL LB-Amp cultures. Incubation takes place at 37C O/N. The problem is the white clones are not growing in the liquid culture whereas the blue clones (negative control) are growing!
I have been trying to obtain my DNA from the white clones for the past week but my starter cultures are not growing!
Can someone please advise and give any suggestions??
Posted 27 August 2010 - 02:08 PM
Posted 27 August 2010 - 07:28 PM
Posted 01 September 2010 - 09:43 AM
A colleague of mine suggested to try transforming into XL10 cells instead of XL2 Blue's.
Does anyone have any experience with these?
Thanks for your suggestions guys
Posted 01 September 2010 - 10:56 AM