fabrablast primery cell line problem
1 reply to this topic
Posted 27 August 2010 - 09:07 AM
i have prepared fibroblast primary cell line from mouse tail. But while i trypsinized the cells, it's not easy to detache them in 175flask. my procedure is
10ml pbs wash,
5ml EDTA-Trypsin, 5min not detached much, then for 10min, still not detached well.
Thanks for your suggestion!
Posted 29 August 2010 - 01:41 PM
Do you incubate them at 37 deg C when you trypsinise the cells? If not, try that and see how it goes. Some cell lines can take a while to come off.