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Retrovirus production


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#1 laurequillo

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Posted 27 August 2010 - 03:40 AM

Hi there!

I was producing lentivirus using the pLKO system (pLKO, pspax2, PMD2G).
Now, I want to know how to produce retrovirus without a packaging cell line. Which plasmids should I use to get the retrovirus? I have some construsts in pBABE, and I guess I can use the PMD2G (VSV-G), but what other plasmid do I need?(because the pspax2 is for lentivirus)

Thanks
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#2 Functional Screens

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Posted 28 August 2010 - 09:58 AM

check Takara or NoLan lab
http://catalog.takar...itid=U100005745
http://www.stanford...._packlines.html

However, I will recommend using packaging cell line for retroviral production.

#3 laurequillo

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Posted 28 August 2010 - 12:31 PM

check Takara or NoLan lab
http://catalog.takar...itid=U100005745
http://www.stanford...._packlines.html

However, I will recommend using packaging cell line for retroviral production.


Thanks a lot. I actually had platinum E and phoenix ampho, but they were not properly frozen, so now I have to wait for the new cells to arrive, and that is why I was checking whether I could produce viruses with transient transfection in the meantime. But it looks like I dont have the plasmids anyway, so I will wait for the cells.

Thanks again!
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

"Im the goddamn batman"




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