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Protein is getting a gel


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#1 joerg

joerg

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Posted 19 July 2002 - 07:49 AM

Hi alltogether,
I expressed several light and heavy chains from rat IgG in E.coli with success. But one light chain is becoming gelly, when I resolve my inclusion body pellet in GuHCl and DTT. Sequence is ok (it is a rat light chain), Stop codon seems to be ok. I expressed it two times, and it is allways the same. I do not have any good idea why.
Thanks in advance




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