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DNA product almost 1500 bp


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#1 ocean_sky83

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Posted 26 August 2010 - 11:50 PM

What percentage of the gel is optimal for pcr product around 1500bp? I used 1.5% and obtain a very slight faint band. Is this due to the percentage of my gel? I have repeated by increasing dna template as well

Please advise
Thanks

#2 christy

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Posted 27 August 2010 - 01:20 AM

0.8 % agarose gel is enough to see the 1500 bp length.




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