Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Voltage Ramping Problems

  • Please log in to reply
No replies to this topic

#1 kadamb



  • Active Members
  • Pip
  • 7 posts

Posted 31 July 2002 - 11:04 PM

Hi! i am doing IEF using Bio-Rad IPG strip 17cm and IEF system, One thing which always bothers me is voltage ramping, once the rehydration is over after placing wicks IEF is started but somehow voltage ramping does not match with the time say from 500Volts to 10000 volts in 6 hours but voltage never reached 10000 even after 6 hours.  What could be problems besides high salts which is not present and rehydration is in standard active rehydration buffer.How do we know time requried for focusing how do we know that this is overfocusing, i can't find anywhere.Eagerly waiting for suggestion and advice.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.