Second question I have is the method for quantification. Normally for cDNA I performed sybr green using two gene specific primers that are 20 bp and generated from primer 3 program (online). What primers do I need for let's say miRNA: 3' cuaAGGUUAAAAAGGUGUAGAa 5' hsa-miR-576-3p??? When they say loop stem primers what exatly do you mean? How do I find the where is the loop portion? Is there a program to help me out?
Any ideas/ advice will be greatly received!!!!
Edited by mfragiad, 25 August 2010 - 07:29 AM.