Hi everybody
I am working on primary cancer cells. My goal is to find if there is a cooperation between my gene, which is mitotic protein, and the mitotic defect in cancer cells. I have done immunefluorescent experiment. I did not find correlation between the protein level and the mitotic defect. My supervisor wants me to do DNA content analysis experiment using FACS on the same sample set to test the correlation between the gene and the percentage of aneuploid.
She said that FACS can look deeply in the cells and might give different result than IF result. What do you think? Does it worth to try FACS?? Please help me am new PhD student
0
2 replies to this topic
#2
Rsm
-
- Active Members
-
- 361 posts
Post Dog
4
Neutral
Neutral
Posted 29 November 2010 - 01:06 AM
A good thing about FACS is also that you can measure 10,000 cells in 20 seconds. With IF you can maybe measure 10... So you get more reliable data. Make sure you have good controls!
If your gene is a cytoplasmic protein, it may be difficult to stain your cells for FACS. But not impossible...
rsm
If your gene is a cytoplasmic protein, it may be difficult to stain your cells for FACS. But not impossible...
rsm
Edited by Rsm, 29 November 2010 - 01:08 AM.
I got soul, but I'm not a soldier
#3
Denis Baev
-
- Active Members
-
- 53 posts
Enthusiast
0
Neutral
Neutral
Posted 19 January 2011 - 07:32 AM
Rsm, on 29 November 2010 - 01:06 AM, said:
A good thing about FACS is also that you can measure 10,000 cells in 20 seconds.
Modern cytometers in use (such as LSR-II, Canto II, Aria) are able to measure up to 20-30000 events/sec with appropriate signal CV% (doing that basically everyday in my core facility)
