Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

what solvent is better compatible for most cell lines?


  • Please log in to reply
3 replies to this topic

#1 moljul

moljul

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 127 posts
2
Neutral

Posted 24 August 2010 - 05:37 AM

the compound which i have to dissolve is soluble in DMSO and methanol. which of those solvents is more compatible for most of the cell lines??



#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,097 posts
57
Excellent

Posted 24 August 2010 - 09:28 AM

DMSO of course, but the amount should be very very low, because DMSO is toxic at the same time.

We dissolved genistin in DMSO to study endocytosis of virus into eukaryotic cells. I don't remember exactly how much but if I find I will let you know.

#3 labrat612

labrat612

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 87 posts
1
Neutral

Posted 24 August 2010 - 10:06 AM

I would agree. DMSO is usually the way to go.
Although, as an experimental condition, if you have the ability and the time, dissolve your substance in DMSO and Methanol to see that there wasn't some other cellular effect that wasn't predicted.

#4 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 302 posts
60
Excellent

Posted 30 August 2010 - 05:45 AM

if you use organic solvents, always run controls with the solvent alone in the concentration used; DMSO should be under 0,5% end concentration




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.