3 replies to this topic

[ocean_sky83]

Hi

May I ask which temperature is most optimal for keep bacteria in fetal calf serum. -70 or -80? I did observe the usual temperature for storage of bacteria is -70 degree celcius... But due to the original factory setting in my freezer I have been using -80 for a long time. I am paranoid whether this is acceptable or whether this will have any effects on my isolates??

Thanks!

Have a good day =)

[pito]

-80 is a temperature that is often used. Especially in stocking cultures in liquid nitrogen.

[newborn]

pito, on 24 August 2010 - 07:37 AM, said:

-80 is a temperature that is often used. Especially in stocking cultures in liquid nitrogen.

That would not be different between -70 or -80. I think glycerol is needed in the preservation of bacteria.

[microgirl]

I store my bacteria at -80 in glycerol - 500 ul 65% glycerol + 500 ul bacterial culture.