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uv vis spectrophotometer


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3 replies to this topic

#1 intan

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Posted 23 August 2010 - 03:42 PM

what is the purposes using uv vis spectrophotometer?
it is necessary to use this equipment after gel filtration??
if i don't know something then i will ask others to help me and make me understand

#2 mdfenko

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Posted 23 August 2010 - 08:44 PM

there are a lot of uses for a uv/vis spectrophotometer.

what i think you are asking is if you need it to find and/or quantify the protein in your fractions and the answer to that is: maybe. it depends on what you already know and what you need to find out and where you are going from there.
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#3 Inmost sun

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Posted 30 August 2010 - 06:06 AM

in general, for instance, to monitor enzyme assays based on the NAD/NADH system; you should check at 260 nm and 340 nm
for maxima of absorption;

as mdfenko already answered to monitor amounts of protein,e.g. integrated in a HPLC/FPLC system...

Edited by Inmost sun, 30 August 2010 - 06:09 AM.


#4 intan

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Posted 01 September 2010 - 07:39 AM

tQ :)
if i don't know something then i will ask others to help me and make me understand




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