Does unautoclaved DEPC inhibit northern blotting?
Posted 23 August 2010 - 12:55 PM
Posted 23 August 2010 - 02:50 PM
Posted 26 August 2010 - 06:43 PM
You need to autoclave it to inactivate the trace DEPC. DEPC will react with anything that has a -N, -O, or -S, so it may screw up your RNA bases. I would autoclave to be safe, especially if you're using it to store RNA in.