Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Quantification of PCR products


  • Please log in to reply
1 reply to this topic

#1 Fra Cicala

Fra Cicala

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 23 August 2010 - 10:48 AM

Hi I should do a quantification of my PCR products but I don't know what kind of buffer can I use as "BLANK".
Some one can help me?
Thanks
Fra Cicala

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,469 posts
247
Excellent

Posted 23 August 2010 - 05:34 PM

You must clean up the pcr reaction prior to quantification. The pcr reaction has excess dNTPs and primers which will falsely read as product. Clean up on a spin column, or quantify by measuring the intensity of the band on a gel (probably best, since you also know the product is the right size).




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.