Hi I should do a quantification of my PCR products but I don't know what kind of buffer can I use as "BLANK".
Some one can help me?
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Quantification of PCR products
1 reply to this topic
Posted 23 August 2010 - 05:34 PM
You must clean up the pcr reaction prior to quantification. The pcr reaction has excess dNTPs and primers which will falsely read as product. Clean up on a spin column, or quantify by measuring the intensity of the band on a gel (probably best, since you also know the product is the right size).