Hello,
I am searching a good protein marker for cytoplasmic contamination in nuclear extracts. I think tubulin is a good one, but I am not sure which one to choose: alpha or beta isoform. Is there any difference using one or another? And What do you think about using GAPDH for this control? Thanks!
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#2
bob1
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Posted 23 August 2010 - 02:53 PM
I use B-tubulin.
#3
nocas
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Posted 24 August 2010 - 02:20 AM
What about beta-actin? I think it may also be suitable for that.
#4
bob1
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Posted 24 August 2010 - 02:23 PM
Actin is found in both cytoplasm and nucleus.
