No band after WB revelation
Posted 23 August 2010 - 01:46 AM
Im doing wb using a HRP coupled secondary antibody and my problem is that when I put the WEST PICCO reagents I actually see brown bands on my PVDF membrane (these are the bands expected) but when Im revealing my membrane in the dark room I dont see anything on my film....!!!!! No one in my entire lab has ever seen a problem like that!!!
ps: I changed the film, the west picco reagents, and the revelation liquid...nothing!
Please your help is really needed.
Posted 23 August 2010 - 04:57 AM
Posted 23 August 2010 - 05:03 AM
"This is SPARTA!"
"I´m the goddamn batman"