20 replies to this topic

[Rupam]

Try LB media and as E.Coli is fastest growing bacteria then provide the media after a particular interval this will increase the amount of bacteria as well as the OD.

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Regards
Rupam

[microbexpert]

Hi

LB Medium or L-Broth typically contain 10 g of tryptone and 5 g of yeast extract per liter, and can vary in salt concentration from 0.5 g to 10 g per liter. This could be the best media choice for growing E.Coli.

Microbexpert

[jazzlynblu]

I grow my E. coli on tryptic soy agar slants, typically prepared such that they are 15-25 degrees slanted. Streak-spreading E. coli on the surface of the slant typically yields 10^10 cfu/ml consistently after 18-24 hours incubation at 30-35 degree celcius.

[Thomas Karmen DDS]

SOB is the rule of thumb for me and we do many anerobes.

[ascacioc]

magnesium (2 mM) in media makes bacteria grow to higher densities

http://www.ncbi.nlm....pubmed/15915565