Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

How to grow more E.coli?


  • This topic is locked This topic is locked
20 replies to this topic

#16 Rupam

Rupam

    member

  • Banned
  • Pip
  • 22 posts
0
Neutral

Posted 05 September 2010 - 09:14 AM

Try LB media and as E.Coli is fastest growing bacteria then provide the media after a particular interval this will increase the amount of bacteria as well as the OD.

You can also refer [url="[url]http://www.wiziq.com/tutorial/39464-biotechnology-and-its-uses-1"]uses[/url] of biotechnology, biotechnology uses[/url] this will surely help you for your all queries.

Regards
Rupam

#17 microbexpert

microbexpert

    member

  • Watched members
  • Pip
  • 2 posts
0
Neutral

Posted 19 December 2010 - 03:00 PM

Hi

LB Medium or L-Broth typically contain 10 g of tryptone and 5 g of yeast extract per liter, and can vary in salt concentration from 0.5 g to 10 g per liter. This could be the best media choice for growing E.Coli.

Microbexpert

#18 jazzlynblu

jazzlynblu

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 January 2012 - 05:58 PM

I grow my E. coli on tryptic soy agar slants, typically prepared such that they are 15-25 degrees slanted. Streak-spreading E. coli on the surface of the slant typically yields 10^10 cfu/ml consistently after 18-24 hours incubation at 30-35 degree celcius.

#19 alexa jones

alexa jones

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 May 2012 - 01:06 AM

there is a lot of medium to grow E.coli but SOB or LB medium are good.

#20 Thomas Karmen DDS

Thomas Karmen DDS

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 20 May 2012 - 07:25 PM

SOB is the rule of thumb for me and we do many anerobes.

#21 ascacioc

ascacioc

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 271 posts
44
Excellent

Posted 30 August 2012 - 12:45 PM

magnesium (2 mM) in media makes bacteria grow to higher densities

http://www.ncbi.nlm....pubmed/15915565




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.