My exam, pls answer
Posted 21 August 2010 - 07:34 AM
other organisms available in the database but with a very low DNA-homology (40%) with each other.
a) What will you do to clone DNA fragments putative harboring geneX?
How will you select E.coli transformant harboring geneX? Suppose there was not suitable protocol for assaying enzymeX.
c) If you want to know whether geneX is expressed in E.coli or not. What will you do?
d) If geneX is not expressed in E.coli, how will you do to get the geneX to express in E.coli transformant?
e) How will you get purified enzyme from the E.coli transformant?
Posted 21 August 2010 - 11:57 AM
Posted 22 August 2010 - 10:44 AM
Edited by adrian kohsf, 22 August 2010 - 10:46 AM.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434