I am very new to siRNA and shRNA. I want to generate the lentivirus coding shRNA for down regulating the DYNLT1 expression.
Here is the DNA sequence of the DYNLT1:
The bold and underlined part is siRNA CCACAAAUGUAGUAGAACA used by PMID: 20690922. They have shown that siRNA can down regulate the DYNLT1 expression in siRNA transfected cells.
My question is can I use the same sequence as sense sequence and create shRNA oligo?
Italics and bold indicates hairpin.
Can above construct be able to downregulate the DYNLT1 expressing?
Thanks a lot for your time and suggestions.
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siRNA sequence can be used as shRNA?
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