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gDNA extraction with pestle and mortar without liquid nitrogen?


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5 replies to this topic

#1 proteinz

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Posted 20 August 2010 - 07:44 AM

Hi all,
i was wondering if anyone has performed a gDNA extraction with pestle and mortar without liquid nitrogen? We do not have a bead beater either..
Any suggestions? could i just freeze sample at -80 grind, thaw repeat?
thanks!

#2 hobglobin

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Posted 20 August 2010 - 08:34 AM

Hi all,
i was wondering if anyone has performed a gDNA extraction with pestle and mortar without liquid nitrogen? We do not have a bead beater either..
Any suggestions? could i just freeze sample at -80 grind, thaw repeat?
thanks!

depending on the material you could try to use dried or freeze-dried material for this...

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#3 proteinz

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Posted 20 August 2010 - 01:26 PM

Thanks for the reply! My sample is a large community of bacteria. Unfortunately I don't have access to a lyophiliser. Any other ideas?

#4 phage434

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Posted 20 August 2010 - 02:44 PM

Freeze the mortar and pestle also, perhaps with some ethanol, and you can keep things at near -80 while you grind them.
You'll need good insulating gloves.

#5 bob1

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Posted 22 August 2010 - 12:57 PM

Or use dry ice as the freezing agent for grinding.

#6 Adrian K

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Posted 22 August 2010 - 06:41 PM

Another alternative for you:
Boil your bacteria colonies in sterile water, briefly spin down the debris and use the supernatant as template.
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