Hi all,
i was wondering if anyone has performed a gDNA extraction with pestle and mortar without liquid nitrogen? We do not have a bead beater either..
Any suggestions? could i just freeze sample at -80 grind, thaw repeat?
thanks!
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gDNA extraction with pestle and mortar without liquid nitrogen?
Started by proteinz, Aug 20 2010 07:44 AM
5 replies to this topic
#2
hobglobin
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Posted 20 August 2010 - 08:34 AM
proteinz, on 20 August 2010 - 07:44 AM, said:
Hi all,
i was wondering if anyone has performed a gDNA extraction with pestle and mortar without liquid nitrogen? We do not have a bead beater either..
Any suggestions? could i just freeze sample at -80 grind, thaw repeat?
thanks!
i was wondering if anyone has performed a gDNA extraction with pestle and mortar without liquid nitrogen? We do not have a bead beater either..
Any suggestions? could i just freeze sample at -80 grind, thaw repeat?
thanks!
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#3
proteinz
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Posted 20 August 2010 - 01:26 PM
Thanks for the reply! My sample is a large community of bacteria. Unfortunately I don't have access to a lyophiliser. Any other ideas?
#4
phage434
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Posted 20 August 2010 - 02:44 PM
Freeze the mortar and pestle also, perhaps with some ethanol, and you can keep things at near -80 while you grind them.
You'll need good insulating gloves.
You'll need good insulating gloves.
#5
bob1
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Posted 22 August 2010 - 12:57 PM
Or use dry ice as the freezing agent for grinding.
#6
Adrian K
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Posted 22 August 2010 - 06:41 PM
Another alternative for you:
Boil your bacteria colonies in sterile water, briefly spin down the debris and use the supernatant as template.
Boil your bacteria colonies in sterile water, briefly spin down the debris and use the supernatant as template.
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
