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Protein TAG for affinity purification


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#1 mgn67

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Posted 20 August 2010 - 03:46 AM

Hi,
I'd like to express proteins and purify them by affinity chromatography, but i don't really know what tag to use. Actually, i would like to tests 4 or 5 different tags, like His-tag, MBP-tag, etc... I've heard of a tag which would be autocleavable in presence of ubiquitin ? Does anyone have any suggestion, any idea on tags working better than others ? My aim is to cristallize those proteins so purification by IP is not the best way to do... so i have to do without FLAG, HA, Myc, V5 or i don't what tags...

Thanks for you help !
Morgan

#2 bob1

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Posted 22 August 2010 - 01:02 PM

His tag is commonly used for purification of proteins using NiNTA beads.




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