Hi,
I've been trying to elute my protein from the M2Flag beads but with no luck.
I tried low pH, different concentration of peptide...and still the same result...the protein comes out only after boiling the beads.
How can I improve the elution?
Thanks
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2 replies to this topic
#2
laurequillo
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Posted 20 August 2010 - 03:13 AM
How did you do the elution, and what concentrations of flag-peptide did you use? it is the 3xFlag or Flag?Hi,
I've been trying to elute my protein from the M2Flag beads but with no luck.
I tried low pH, different concentration of peptide...and still the same result...the protein comes out only after boiling the beads.
How can I improve the elution?
Thanks
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#3
perionix
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Posted 20 August 2010 - 03:32 AM
Elution: peptide (Sigma): 200ug/ml- 30 min at 4°C..one elution....
Than I tried to increase the elution numbers...and even the concentration up to 1mgr/ml....the result was the same....
I have 1xFLAG
Than I tried to increase the elution numbers...and even the concentration up to 1mgr/ml....the result was the same....
I have 1xFLAG
