GFP fused proteins
Posted 19 August 2010 - 11:06 PM
I'm trying to use GFP as a reporter gene to show arsenic in E.coli.
I cloned GFP after another gene which has a sensitive promoter to arsenic and expected to see fluorscent bacteria after adding arsenic compounds to media. but the results were not be good.the expression of GFP is not enough.i repeated the the experiment again and again...and ...nothing!!!
i'm really upset. i think the genes sequences are ok and the problem is about the expression.help me pls
Posted 20 August 2010 - 04:18 AM
Did you fuse the GFP before the stop codon of the first gene?
Was the fusion integrated into the genome or was it expressed from a plasmid?
Posted 20 August 2010 - 05:51 AM
Posted 24 August 2010 - 09:03 PM