Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

About Laminin 5'UTR 's function


  • Please log in to reply
1 reply to this topic

#1 mickey

mickey

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 19 August 2010 - 01:45 AM

Dear guys, I know many of you are great masters of cloning. I have a question, I read about some literature that lamin A 5' UTR will increase expression in mammalian expression vector. How does it happen? Because I found some company sold "pure" ORF plasmid, is it OK just ORF ready for transfection?

I wanna know the mechanism of the great job of lamin A 5'UTR, thank you for reading my topic !!

#2 Rsm

Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 384 posts
9
Neutral

Posted 19 August 2010 - 03:02 AM

AFAIK, all mammalian expression vectors contain a 5'UTR upstream of the MCS. So you can clone you ORF directly, and it will be provided with a UTR automatically. But I'm not a real clone geek...


Best,

rsm
I got soul, but I'm not a soldier




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.