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About Laminin 5'UTR 's function

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#1 mickey



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Posted 19 August 2010 - 01:45 AM

Dear guys, I know many of you are great masters of cloning. I have a question, I read about some literature that lamin A 5' UTR will increase expression in mammalian expression vector. How does it happen? Because I found some company sold "pure" ORF plasmid, is it OK just ORF ready for transfection?

I wanna know the mechanism of the great job of lamin A 5'UTR, thank you for reading my topic !!

#2 Rsm


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Posted 19 August 2010 - 03:02 AM

AFAIK, all mammalian expression vectors contain a 5'UTR upstream of the MCS. So you can clone you ORF directly, and it will be provided with a UTR automatically. But I'm not a real clone geek...


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