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how to prepare garlic extract for GC?


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#1 ikwana

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Posted 18 August 2010 - 09:59 PM

how to prepare extraction of garlic before undergoe Gas chromatography?

is this correct?

Preparation of extracts:
Fresh garlic cloves were peeled, coarsely chopped with a knife and a 10 g sample homogenised for 2 min with 100 ml distilled water in a food blender (Kenwood). The homogenate was allowed to stand at room temperature for 15 min to ensure the complete enzymatic transformation of thiosulphinates before being filtered through 4 layers of cheesecloth.
A 50 ml sample of the filtrate was pipetted to a 500 ml conical flask and 15 g sodium chloride (NaCl) added to saturate the solution. After ensuring complete dissolution of the NaCl, 50 ml cold dichloromethane (CH2Cl2) was added and the stoppered flask agitated vigorously for 3 min. The resultant emulsion was centrifuged at 4000 rpm for 15 min, the lower solvent layer removed and dried twice with anhydrous sodium sulphate (Na2SO4).
A 10 ml sample of the dry extract was then pipetted to a 10 l measuring cylinder. This was placed in a water bath at 25C 5C, and the extract was rapidly concentrated by bubbling dry nitrogen through it. The solvent was evaporated to < 1 ml final volume and then adjusted accurately to 1 ml with dichloromethane. The concentrated extract was taken up in a glass syringe and injected directly through a 0.2 m in-line nylon filter into a 10 m fixed sample loop.
Due to the instability of thiosulphinates in organic solvents all HPLC runs were undertaken within 45 min of extraction.

#2 johnmartin

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Posted 11 March 2011 - 01:55 AM

Thank you for information ! I was looking for this preparation recepie form long time but didn't get any where..You written everything in a very simple language..I will certainly go for it..
irrigation systems design




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