2 replies to this topic

[huh]

Hello All.

I am running a protein on a BCA Protein Assay (Pierce) and getting good results.  I loaded 20 uL of the undiluted sample (approx 2ug/uL according to BCA, so 40 ug)on a denaturing 4-20% Novex SDS-PAGE gel with 5 uL sample buffer and see absolutely nothing.  Any suggestions/insight would be greatly appreciated.

[Chiapet874]

I dunno what concentration sample buffer you are using, but usually I use a 1:1 volume ratio of sample buffer to diluted/undiluted sample

And what do you mean by "See absolutely nothing"? You mean you don't see the protein lanes running down or something?

[K.B.]

Have you fixed your gel before staining? What kind of staining have you used?