Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

BCA Assay and SDS-PAGE Gels Do Not Agree


  • Please log in to reply
2 replies to this topic

#1 huh

huh

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 18 August 2010 - 12:11 PM

Hello All.

I am running a protein on a BCA Protein Assay (Pierce) and getting good results. I loaded 20 uL of the undiluted sample (approx 2ug/uL according to BCA, so 40 ug)on a denaturing 4-20% Novex SDS-PAGE gel with 5 uL sample buffer and see absolutely nothing. Any suggestions/insight would be greatly appreciated.

#2 Chiapet874

Chiapet874

    member

  • Active Members
  • Pip
  • 21 posts
0
Neutral

Posted 18 August 2010 - 07:15 PM

I dunno what concentration sample buffer you are using, but usually I use a 1:1 volume ratio of sample buffer to diluted/undiluted sample

And what do you mean by "See absolutely nothing"? You mean you don't see the protein lanes running down or something?

#3 K.B.

K.B.

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 174 posts
3
Neutral

Posted 19 August 2010 - 05:20 AM

Have you fixed your gel before staining? What kind of staining have you used?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.