Making gels in advance
Posted 18 August 2010 - 09:59 AM
Posted 18 August 2010 - 10:03 AM
Part of this, I think is personal preference. But, we felt that gels can dry out over time. Therefore, 1-2 days carefully wrapped up in the cold room would be our maximum.
So for us, we've instituted the over-night rule: Figure out the number of gels you think you would run the next day, and prepare them at least the night before.
And if you can't use them, then do your best to run them the day after.
Again, that's just our preference. So far, it's worked for us.
Posted 19 August 2010 - 02:58 AM
I usually prepare 20-30 gels once a month and store them in TBE buffer (or whatever buffer you use for gels) at 4C in a tupper box. You can obviously not store them on air or wrapped in saran wrap, they will dry out. For me storage works well since many years. They are stable for at least 4 weeks, when you use EtBr (haven't tried other dyes, and won't).