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L-form staining procedures

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#1 NBaye



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Posted 19 June 2002 - 06:45 AM

I have just begun to work with stable cell-wall-deficient bacteria (L-forms).  The Acridine Orange staining procedure that I have obtained from _ Specific Procedures and Requirements for the Isolation, Growth and Maintenance of the L-Phase of some Microbial Groups _ is fixed with Bouin's fixative.  I was wondering if any other fixative exists (that would be appropriate for this work) that does not contain Picric Acid.

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