I have just begun to work with stable cell-wall-deficient bacteria (L-forms). The Acridine Orange staining procedure that I have obtained from _ Specific Procedures and Requirements for the Isolation, Growth and Maintenance of the L-Phase of some Microbial Groups _ is fixed with Bouin's fixative. I was wondering if any other fixative exists (that would be appropriate for this work) that does not contain Picric Acid.
L-form staining procedures
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