2 replies to this topic

[YVETTE]

Dear fellows,

I am planning to do some experiment on the regulation of NF-kappaB subunit on some proinflammatory gene such as IL-8 and IL-6. I will do ChIP to see if NF-kappaB subunit such as p50 RelB bind on the promoter of IL-8 promoter. First of all I have to find the sequence of the binding site. I wanna know if there are any bioinformatic tools to do this job. thanks

[bob1]

If you can find a consensus binding sequence then you can search for that sequence in promoter regions of the genes.  I think there is a site called TFbind or something similar that has a lot of the binding sites for transcription factors.

[laurequillo]

Yeah, Bob is right, and for NFkB there is a known consensus site which you can find in your promoters even manually

Edited by laurequillo, 18 August 2010 - 10:48 PM.