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How to extract restriction enzyme (or restriction endonuclease) from bacteria?


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#1 draggy

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Posted 18 August 2010 - 05:02 AM

Hello People,

Is there a simple method to extract restriction enzyme from bacteria?

Thank you.

#2 protolder

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Posted 06 September 2010 - 09:59 PM

Hola, Im not sure but I supposse that if you could , you will obtain an small amount. I think that they are obtained by cloning , probably with a tag to achieve a big amount of active pure protein. Regards




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