2 replies to this topic
Posted 24 September 2002 - 04:58 AM
you can do it, but eg. for bacterial freezing stocks it is not necessary.
Posted 27 December 2002 - 01:47 PM
Glycerol solutions intended for preparation of electro-competent bacteria should be filtered rather than autoclaved for higher transformation efficiency. Glycerol will start to decompose to ethylene aldehyde at its boiling point of 182°C.