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#1 sam

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Posted 07 August 2002 - 12:32 PM

Can you sterilise glycerol by autoclaving? If not why?

#2 snowman

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Posted 24 September 2002 - 04:58 AM

you can do it, but eg. for bacterial freezing stocks it is not necessary.

#3 tfitzwater

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Posted 27 December 2002 - 01:47 PM

Glycerol solutions intended for preparation of electro-competent bacteria should be filtered rather than autoclaved for higher transformation efficiency. Glycerol will start to decompose to ethylene aldehyde at its boiling point of 182C.




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