2 replies to this topic

[sam]

Can you sterilise glycerol by autoclaving? If not why?

[snowman]

you can do it, but eg. for bacterial freezing stocks it is not necessary.

[tfitzwater]

Glycerol solutions intended for preparation of electro-competent bacteria should be filtered rather than autoclaved for higher transformation efficiency.  Glycerol will start to decompose to ethylene aldehyde at its boiling point of 182°C.