Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNAse digestion while RNA extraction with TRIzol


  • Please log in to reply
1 reply to this topic

#1 Johannes

Johannes

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 17 August 2010 - 05:52 AM

Hi,

a colleague of mine needs to do a DNAse digestion on her RNA. We are now looking for simple ways of including the DNAse step into the TRIzol RNA extraction protocol.

I was thinking about including it after taking the aqueous phase containing the RNA like that:

- take RNA phase and put it in a new cup
- add DNAse and DNAse buffer
- incubate

and then to inactivate the DNAse i would start all over with the TRIzol protocol with a smaller amount of TRIzol.

What do you think about that?

cheers
Johannes

#2 NemomeN007

NemomeN007

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 34 posts
0
Neutral

Posted 17 August 2010 - 10:57 AM

I'm not sure how well the DNAse will work in the presence of chaotropic salts. But, as I teach and do, just try and see what you get.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.