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DNA-binding protein(s)

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#1 efthalia



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Posted 13 February 2002 - 04:47 PM

I am looking for techniques to analyze specific DNA binding of a putative transcription activator. I need to be able to identify it, isolate it, and determine what other factors may be interacting with it.  Can anyone describe a biotin-strepavidin method to isolate the putative binding-site once it has been incubated with cell lysate?

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