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What is the BGH gene for in a expression vector?


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#1 wu xin

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Posted 16 August 2010 - 12:59 PM

Hi,

Could anyone tell me what's the BGH gene for in a expression vector, please?

Many thanks!

wu xin

#2 bob1

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Posted 16 August 2010 - 03:05 PM

Are you sure it is the full BGH, not just a primer binding site or a polyadenylation signal?

#3 wu xin

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Posted 20 August 2010 - 07:40 AM

View Postbob1, on 16 August 2010 - 03:05 PM, said:

Are you sure it is the full BGH, not just a primer binding site or a polyadenylation signal?

many thanks for your reply!  It's about 230 bp long and seems like poly A signal to me.

#4 bob1

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Posted 22 August 2010 - 01:09 PM

It is there as a poly A tail generator for the mRNA made off the plasmid, this helps with message processing in protein production.

#5 wu xin

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Posted 02 September 2010 - 02:53 PM

View Postbob1, on 22 August 2010 - 01:09 PM, said:

It is there as a poly A tail generator for the mRNA made off the plasmid, this helps with message processing in protein production.


Many thanks bob!!!

wu xin




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