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HpaI blunt end cloning


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#1 solcole

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Posted 16 August 2010 - 09:46 AM

Hi all,

Just a doubt. When HpaI cuts DNA of interest, it generates a blunt end. I just want to check if the blunt end has a phosphate group in order for ligation to occur with a vector (also cut with HpaI)?

#2 NemomeN007

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Posted 16 August 2010 - 09:53 AM

Yes it does...watch out for recircularization during ligation.




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