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storing oligos


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#1 aburke

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Posted 09 April 2002 - 01:26 PM

Hi.  Just wondering if anyone can tell me what the best way to aliqout and dry oligonucleotide samples for long-term storage is.  I am planning on dissolving them in TE buffer, but I don't know how I should go about drying the solution for -20C storage.

#2 Frederic

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Posted 11 April 2002 - 01:24 AM

Oligo's (primers) can be disolved either in DEPC water or in TE and stabely stored at -20C

#3 Diane Dutt

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Posted 27 June 2002 - 12:50 PM

Typically I distribute my oligos as 100 ul volumes in microfuge tubes, dry them down in a Spin-Vac (Savant) and store the dehydrated oligos at -80 deg.  To use the oligos, I bring them up in 100 ul distilled and store them at 4 deg.

#4 Diane Dutt

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Posted 27 June 2002 - 12:51 PM

Typically I distribute my oligos as 100 ul volumes in microfuge tubes, dry them down in a Spin-Vac (Savant) and store the dehydrated oligos at -80 deg.  To use the oligos, I bring them up in 100 ul distilled and store them at 4 deg.




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