Hi everyone,
can any one tell me how i can get my gene of interest back after having insertion in T/A cloning vector (like pTZ57R/T) as I have no place any restriction sites in gene while doing amplification?
awaiting
af malik
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1 reply to this topic
#2
Adrian K
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Legendary Graduate Beggar
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Excellent
Excellent
Posted 14 August 2010 - 05:33 AM
Do a pcr using the primers that you had cloned your insert, and the cloned vector as your template. is this what you want?
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
