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how i can get insert back?


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#1 af malik

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Posted 14 August 2010 - 01:31 AM

Hi everyone,

can any one tell me how i can get my gene of interest back after having insertion in T/A cloning vector (like pTZ57R/T) as I have no place any restriction sites in gene while doing amplification?

awaiting
af malik

#2 Adrian K

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Posted 14 August 2010 - 05:33 AM

Do a pcr using the primers that you had cloned your insert, and the cloned vector as your template. is this what you want?
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