I am currently doing some in vitro kinase assays. I IP the kinase with anti_HA coupled beads. But I always had the same problem that the IPed kinase amoount is not always the same among samples even I normalize total protein amount. My question is; after IPing my kinase can I freeze it at -80C or keep it in 4C with protease inhibitors. then I will be able to normalize my kinase amount and run the kinase reaction with the same kinase amounts.
freezing IP before in-vitro kinase assay?
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