Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to plate cells precisely


  • Please log in to reply
1 reply to this topic

#1 dangmytr

dangmytr

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 August 2010 - 07:11 AM

hi everyone,

are there any methods that you guys use to plate cells precisely - so that the number of cells/well are similar amongst each-other with minimal variation?

p.s. sorry for posting this in the wrong folder :(

Edited by dangmytr, 13 August 2010 - 07:21 AM.


#2 labrat612

labrat612

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 87 posts
1
Neutral

Posted 13 August 2010 - 10:52 AM

First, your initial counts should be done at least twice following a slight vortex as cells can sometimes clump.
Secondly, as you're plating, if you're doing really high volumes, some people keep their cells on a magnetic spin plate. If you're doing some low volumes, constantly (but gently) pipet your cells up and down so that they are mixed properly.


Hope that helps!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.