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No protein spots in 2D gel - Silver staining!


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17 replies to this topic

#16 rachelhauser

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Posted 26 August 2010 - 12:23 AM

YuFang 7, I will process by both 1D and 2D SDS-Page... I have no idea what proteins exist in my samples. So, I'll follow your suggestions and tell you what happened, if anything! =) Thank you so much!
"Live Long and Prosper".

#17 yufang7

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Posted 26 August 2010 - 04:14 PM

Best of luck with your experiments.

#18 mdfenko

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Posted 26 August 2010 - 10:39 PM

Actually the dye is bromophenol blue, and is included in the rehydration solution in which I rehydrate my IPG strips... The dye should migrate to the + of the strip, even though proteins might reach their pI later... It is proff that a current is passing through the strip...!


in that case you can run the first dimension longer. since you are using an immobilized pH gradient you won't have to worry about cathode drift.
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