No protein spots in 2D gel - Silver staining!
Posted 26 August 2010 - 12:23 AM
Posted 26 August 2010 - 10:39 PM
Actually the dye is bromophenol blue, and is included in the rehydration solution in which I rehydrate my IPG strips... The dye should migrate to the + of the strip, even though proteins might reach their pI later... It is proff that a current is passing through the strip...!
in that case you can run the first dimension longer. since you are using an immobilized pH gradient you won't have to worry about cathode drift.
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