Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

No protein spots in 2D gel - Silver staining!

  • Please log in to reply
17 replies to this topic

#16 rachelhauser



  • Active Members
  • Pip
  • 19 posts

Posted 26 August 2010 - 12:23 AM

YuFang 7, I will process by both 1D and 2D SDS-Page... I have no idea what proteins exist in my samples. So, I'll follow your suggestions and tell you what happened, if anything! =) Thank you so much!
"Live Long and Prosper".

#17 yufang7



  • Active Members
  • Pip
  • 8 posts

Posted 26 August 2010 - 04:14 PM

Best of luck with your experiments.

#18 mdfenko


    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,102 posts

Posted 26 August 2010 - 10:39 PM

Actually the dye is bromophenol blue, and is included in the rehydration solution in which I rehydrate my IPG strips... The dye should migrate to the + of the strip, even though proteins might reach their pI later... It is proff that a current is passing through the strip...!

in that case you can run the first dimension longer. since you are using an immobilized pH gradient you won't have to worry about cathode drift.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.