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Deactivation of RBCs from Whole Blood?


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#1 rnazitto

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Posted 12 August 2010 - 01:10 PM

I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!

#2 Clare

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Posted 12 August 2010 - 01:14 PM

Hello :) There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.
If no one else posts a recipe tonight, I'll post it tomorrow :D

Clare

I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!



#3 rnazitto

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Posted 12 August 2010 - 01:24 PM

Thank you!!


Hello :) There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.
If no one else posts a recipe tonight, I'll post it tomorrow :D

Clare


I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!



#4 Clare

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Posted 13 August 2010 - 01:37 AM

here you go: my RBC lysis solution
0.2M NH4Cl
0.01M NaHCO3
0.13mM EDTA

I would usually resuspend the cells in the RBC lysis solution, leave it for a few minutes on ice. Then, either spin down the cells and resuspend in fresh PBS etc. Or add some PBS before spinning down.

Any questions, just ask :)

Clare




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