I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!
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Clare
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Posted 12 August 2010 - 01:14 PM
Hello 
There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.
If no one else posts a recipe tonight, I'll post it tomorrow
Clare
There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.If no one else posts a recipe tonight, I'll post it tomorrow
Clare
rnazitto, on 12 August 2010 - 01:10 PM, said:
I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!
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rnazitto
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Posted 12 August 2010 - 01:24 PM
Thank you!!
Clare, on 12 August 2010 - 01:14 PM, said:
Hello There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.
If no one else posts a recipe tonight, I'll post it tomorrow
Clare
There are commercially available RBC lysis solutions - I make a home made version, but I have the recipe at work. I use it to get rid of RBC in whole blood and BM.If no one else posts a recipe tonight, I'll post it tomorrow
Clare
rnazitto, on 12 August 2010 - 01:10 PM, said:
I was wondering if there was any way to deactivate RBC's from whole blood and not effect any other cell types significantly. I would try lyse buffer with Ammonium Chloride but I was thinking more of blocking RBC receptors or something similar. If I had to lyse I would but again, I dont want to damage anything else significantly other than that RBCs. Thanks!
#4
Clare
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Posted 13 August 2010 - 01:37 AM
here you go: my RBC lysis solution
0.2M NH4Cl
0.01M NaHCO3
0.13mM EDTA
I would usually resuspend the cells in the RBC lysis solution, leave it for a few minutes on ice. Then, either spin down the cells and resuspend in fresh PBS etc. Or add some PBS before spinning down.
Any questions, just ask
Clare
0.2M NH4Cl
0.01M NaHCO3
0.13mM EDTA
I would usually resuspend the cells in the RBC lysis solution, leave it for a few minutes on ice. Then, either spin down the cells and resuspend in fresh PBS etc. Or add some PBS before spinning down.
Any questions, just ask
Clare
