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Selecting Lethal Mutants?


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#1 TomH

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Posted 12 August 2010 - 08:32 AM

Hi all, I was wondering if you could lend your brain power! I'm trying to think of a way of selecting lethal mutations in a randomly transposon mutated bacterial sample. The transposon would contain a resistance cassette for selection of mutated bacteria, however the lethal mutations are probably gonna be the most interesting ones and am trying to think of a way to be able to see what genes have been disrupted even though, by definition, I can't proliferate my lethally mutated bacteria.

I can't see personally how it could be done but thought maybe the bioforum boffins would be able to prove me wrong!

#2 bob1

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Posted 12 August 2010 - 12:31 PM

Conditional selection? i.e. have some system whereby the mutations are not lethal unless turned on? I don't really see how this can be done with a whole genome system though.




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