Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Selecting Lethal Mutants?

  • Please log in to reply
1 reply to this topic

#1 TomH



  • Active Members
  • Pip
  • 12 posts

Posted 12 August 2010 - 08:32 AM

Hi all, I was wondering if you could lend your brain power! I'm trying to think of a way of selecting lethal mutations in a randomly transposon mutated bacterial sample. The transposon would contain a resistance cassette for selection of mutated bacteria, however the lethal mutations are probably gonna be the most interesting ones and am trying to think of a way to be able to see what genes have been disrupted even though, by definition, I can't proliferate my lethally mutated bacteria.

I can't see personally how it could be done but thought maybe the bioforum boffins would be able to prove me wrong!

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,133 posts

Posted 12 August 2010 - 12:31 PM

Conditional selection? i.e. have some system whereby the mutations are not lethal unless turned on? I don't really see how this can be done with a whole genome system though.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.