1 reply to this topic

[TomH]

Hi all, I was wondering if you could lend your brain power! I'm trying to think of a way of selecting lethal mutations in a randomly transposon mutated bacterial sample. The transposon would contain a resistance cassette for selection of mutated bacteria, however the lethal mutations are probably gonna be the most interesting ones and am trying to think of a way to be able to see what genes have been disrupted even though, by definition, I can't proliferate my lethally mutated bacteria.

I can't see personally how it could be done but thought maybe the bioforum boffins would be able to prove me wrong!

[bob1]

Conditional selection?  i.e. have some system whereby the mutations are not lethal unless turned on?  I don't really see how this can be done with a whole genome system though.