1 reply to this topic

[hotstuffdb22]

Hello,

I have an assay where I digest all chromosomes with two generic primers and then place the digested samples on a Southern blot for visualization to look at size differences.  However, I am unable to identify which chromosomes have a particular size.  Are there any enzymes that are specific for individual chromosomes, such that they cut only one chromosome and not any other? (i.e. - a single cutter)

Thanks!

Edited by hotstuffdb22, 12 August 2010 - 06:41 AM.

[bob1]

Southern blots do not give chromosomal localisation, you need to do FISH (fluorescent in situ hybridisation) or a similar technique.  The probes you use for the Southern should work for this.

Edited by bob1, 12 August 2010 - 12:36 PM.