I have an assay where I digest all chromosomes with two generic primers and then place the digested samples on a Southern blot for visualization to look at size differences. However, I am unable to identify which chromosomes have a particular size. Are there any enzymes that are specific for individual chromosomes, such that they cut only one chromosome and not any other? (i.e. - a single cutter)
Edited by hotstuffdb22, 12 August 2010 - 06:41 AM.