Hi everyone,
I wanna extract RNA from cell that must be fixed before due to staining for cell sorting
But as we have known that most of fixative cause the low A260/230
And unfortunately, these RNA are required to perform the microarray analysis
Can anyone teach me how to preserve the integrity of RNA in fixed cells
Thanks for the answers
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2 replies to this topic
#2
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Hmmm, I think it's working
Posted 12 August 2010 - 12:33 PM
What are the cells fixed in? The fixative will determine the method and the effectiveness of the extraction.
#3
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Posted 15 August 2010 - 01:54 PM
I'm actually curious about this as well. We usually fix our cells in 3.7-4% PFA, wouldn't the actual staining process affect the integrity of the RNA as well?
