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RNA extraction from stained cells


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#1 shally

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Posted 11 August 2010 - 08:43 PM

Hi everyone,

I wanna extract RNA from cell that must be fixed before due to staining for cell sorting

But as we have known that most of fixative cause the low A260/230

And unfortunately, these RNA are required to perform the microarray analysis

Can anyone teach me how to preserve the integrity of RNA in fixed cells

Thanks for the answers

#2 bob1

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Posted 12 August 2010 - 12:33 PM

What are the cells fixed in? The fixative will determine the method and the effectiveness of the extraction.

#3 TimUCR

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Posted 15 August 2010 - 01:54 PM

I'm actually curious about this as well. We usually fix our cells in 3.7-4% PFA, wouldn't the actual staining process affect the integrity of the RNA as well?




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