2 replies to this topic

[shally]

Hi everyone,

I wanna extract RNA from cell that must be fixed before due to staining for cell sorting

But as we have known that most of fixative cause the low A260/230

And unfortunately, these RNA are required to perform the microarray analysis

Can anyone teach me how to preserve the integrity of RNA in fixed cells

Thanks for the answers

[bob1]

What are the cells fixed in?  The fixative will determine the method and the effectiveness of the extraction.

[TimUCR]

I'm actually curious about this as well. We usually fix our cells in 3.7-4% PFA, wouldn't the actual staining process affect the integrity of the RNA as well?