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Microbial esterase assay

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#1 momcisrb



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Posted 25 May 2002 - 06:09 AM

I am looking for a quick and easy method of quantifying microbial esterases.
I have tried fluorescein diacetate but have had a lot of solubility problems, if anyone knows of a method with this compound it would be appreciated.
I have also tried para-nitrophenol compounds but they seem to lack the specificity for microbial esterases.
Any suggestions gratefully recieved.

#2 Dr.B



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Posted 24 November 2004 - 02:12 AM

I used para-nitrophenol compounds (C2-C12) and found huge differences in specificity for some esterases.
A simple old fashioned method is preparing plates containing 3ml of an emulsion of 50% tributyrin and 5% gum arabic per 100 ml of medium. You will get turbid plates, grwoth of esterase containing bacteria will form a clear halo around the colony.

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