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IHC nuclear staining


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#1 SF_HK

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Posted 11 August 2010 - 07:00 AM

I'm new to IHC staining and I woul dlike to check the localization of b-catenin (whether its nuclear or cytoplasmic or both). I'm doing IHC staiing with the antibody folowed by hemotoxylin staining? Is this correct or do I have to do H&E staining to see the cytoplasmic and nuclear staining both?

Thanks
SF

#2 bob1

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Posted 11 August 2010 - 02:17 PM

You should do both, otherwise you will be unable to determine the extent of the cytoplasm, in case you find it in the cytoplasm as well as the nucleus.

#3 Clare

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Posted 12 August 2010 - 01:06 AM

Hello :)

Are you going to try an antigen retrieval step for the nuclear staining? I had to do this for the antibody I produced - so just a warning ;)
Clare

I'm new to IHC staining and I woul dlike to check the localization of b-catenin (whether its nuclear or cytoplasmic or both). I'm doing IHC staiing with the antibody folowed by hemotoxylin staining? Is this correct or do I have to do H&E staining to see the cytoplasmic and nuclear staining both?

Thanks
SF






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