2 replies to this topic

[SF_HK]

I'm new to IHC staining and I woul dlike to check the localization of b-catenin (whether its nuclear or cytoplasmic or both). I'm doing IHC staiing with the antibody folowed by hemotoxylin staining? Is this correct or do I have to do H&E staining to see the cytoplasmic and nuclear staining both?

Thanks
SF

[bob1]

You should do both, otherwise you will be unable to determine the extent of the cytoplasm, in case you find it in the cytoplasm as well as the nucleus.

[Clare]

Hello

Are you going to try an antigen retrieval step for the nuclear staining? I had to do this for the antibody I produced - so just a warning
Clare

SF_HK, on 11 August 2010 - 07:00 AM, said:

I'm new to IHC staining and I woul dlike to check the localization of b-catenin (whether its nuclear or cytoplasmic or both). I'm doing IHC staiing with the antibody folowed by hemotoxylin staining? Is this correct or do I have to do H&E staining to see the cytoplasmic and nuclear staining both?

Thanks
SF