IHC nuclear staining
Posted 11 August 2010 - 07:00 AM
Posted 11 August 2010 - 02:17 PM
Posted 12 August 2010 - 01:06 AM
Are you going to try an antigen retrieval step for the nuclear staining? I had to do this for the antibody I produced - so just a warning
I'm new to IHC staining and I woul dlike to check the localization of b-catenin (whether its nuclear or cytoplasmic or both). I'm doing IHC staiing with the antibody folowed by hemotoxylin staining? Is this correct or do I have to do H&E staining to see the cytoplasmic and nuclear staining both?