low yeild fo RNA after invitro transcription
Posted 11 August 2010 - 03:50 AM
Iam getting very low yeild after invitro trascription using T7. I have checked the purity of the DNA, and has been completely linearised. Followed the protocol, did ethanol purification before adding the polymerase and then added the required contents and kept in 37 deg of 2 hr for one and 4 hr for another, and did Lithium Cl ppption and quantified. The yeild was around 77ng/ ul. I dont know why I get a very low yeild. Needed help to over come this problem. Any help will be appreciated.
Posted 11 August 2010 - 09:21 AM
Posted 11 August 2010 - 09:55 PM
Posted 12 August 2010 - 07:26 AM