2 replies to this topic

[newborn]

There are some efficient magnetic beads coated with Protein A Or Protein G for Immunoprecipitation. However, I read somewhere said that C1q should be used in the measurement of human immune complex.

Do you have any information about this?

[Rupam]

Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

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Regards
Rupam Mittal

Edited by Rupam, 24 August 2010 - 10:06 PM.

[newborn]

Rupam, on 24 August 2010 - 03:29 PM, said:

Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

For further queries: biology test papers

Regards
Rupam Mittal

Thanks for your reply, do you think which protein (C1q, Protein A or Protein G) should be used to precipitate immune complex?