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c1q ? Protein A? Or Protein G for immune complex detection?


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#1 newborn

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Posted 10 August 2010 - 07:38 PM

There are some efficient magnetic beads coated with Protein A Or Protein G for Immunoprecipitation. However, I read somewhere said that C1q should be used in the measurement of human immune complex.

Do you have any information about this?

#2 Rupam

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Posted 24 August 2010 - 03:29 PM

Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

For further queries: [url="[url]http://www.wiziq.com/tests/biology"]biology[/url] test papers[/url]

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Rupam Mittal

Edited by Rupam, 24 August 2010 - 10:06 PM.


#3 newborn

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Posted 26 August 2010 - 06:36 PM

Ya c1q can be used for the measurement of human immune complex because C1q-bearing immune complexes induce IL-8 secretion in human umbilical vein endothelial cells (HUVEC) through protein tyrosine kinase- and mitogen-activated protein kinase-dependent mechanisms: evidence that the 126 kD phagocytic C1q receptor mediates immune complex activation of HUVEC

For further queries: http://www.wiziq.com/tests/biology

Regards
Rupam Mittal


Thanks for your reply, do you think which protein (C1q, Protein A or Protein G) should be used to precipitate immune complex?




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